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KMID : 0604520140400020195
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Journal of the Society of Cosmetic Scientists of Korea
2014 Volume.40 No. 2 p.195 ~ p.201
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Effect of Cosmetics Contained Isotonic Water Mimicked Body Fluid on Cell Activities and Skin
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Park Sun-Young
Lee Sung-Hoon
Kim Eun-Joo
Choi So-Woong
Kim Ji-Yeong
Cho Seong-A
Cho Jun-Cheol
Lee Hae-Kwang
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Abstract
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Body fluid has been studied for diverse fields like Ringer’s solutions, artificial joint fluids, cell growth culture media because it plays a crucial role in controlling body temperature and acts as a solvent for diverse metabolite processes in the body and delivery media of mineral, energy source, hormone, signal and drug from and to cell via blood or lymphatic vessel by osmotic pressure or active uptake. Stratum corneum containing extracellular lipids and NMF (natural moisturizing factor) absorbs atmospheric water residing outside of cells and utilize it to hydrate inside of their own. This process is related to skin barrier function. In this study, we conducted the cell viability test with Cell Bio Fluid SyncTM , which mimicks body fluids including amino acids, peptides, and monosaccharides to strengthen skin barrier, and the clinical skin improvement test with cosmetics containing Cell Bio Fluid SyncTM. In the cell viability test, HaCaT cell was treated with PBS for 3 hours, followed by the treatment of a cell culture medium (DMEM) and isotonic solution (PBS) and Cell Bio Fluid SyncTM for 3 hours each. Then, MTT assay and image analysis were conducted. In the clinical skin improvement test, twenty-one healthy women participated. Participants applied cosmetics containing Cell Bio Fluid SyncTM on their face for a week and evaluated the skin hydration, skin roughness, brightness and evenness. All measurements were conducted after they washed off their face and took a rest under the constant temperature (22 ± 2 ℃) and constant humidity conditions (50 ± 5%) for 20 minutes. All the data were analyzed by SPSS (version 21) software program.Results showed that Cell Bio Fluid SyncTM improved both the cell viability and in vivo skin conditions such as skin hydration, roughness, brightness and evenness.
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KEYWORD
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Cell Bio Fluid SyncTM, body fluid, MTT test, clinical study
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